vendredi 24 avril 2009
par   G. Grégori

Boye E, Steen HB, Skarstad K (1983) Flow cytometry of bacteria : a promising tool in experimental and clinical microbiology. J Gen Microbiol 129 :973-980


The DNA and protein content of individual Escherichia coli cells were measured at a rate of 10(4) cells per second with a sensitive microscope-based flow cytometer. DNA and protein were quantified by measuring the fluorescence from cells stained with a combination of the DNA-binding drugs Mithramycin and ethidium bromide and by scattered light, respectively. Separate experiments demonstrated that the light scatter signal was proportional to protein content. Dual parameter histograms (fluorescence/scattered light) of bacterial cultures gave detailed pictures of changes dependent upon the growth conditions and of the cell cycle kinetics. Effects of antibiotics could be readily detected and characterized after a few hours. The results demonstrate that flow cytometry is a promising method for application in experimental and clinical microbiology.

Groschel DH (1983) Methods, reagents and media : new technology in clinical microbiology. Infect Control 4 :232-233


Sahar E, Lamed R, Ofek I (1983) Rapid identification of Streptococcus pyogenes by flow cytometry. Eur J Clin Microbiol 2 :192-195


Flow cytometry combined with immunofluorescence of Streptococcus pyogenes was used to assay bacteria suspended in buffer solution and in saliva derived from throat swabs of healthy volunteers. The method allowed the enumeration of as few as 5 X 10(3) and 5 X 10(4) CFU per milliliter of buffer and saliva respectively. Controls including Streptococcus salivarius instead of Streptococcus pyogenes or buffer instead of specific antibodies confirmed the specificity of the detection of Streptococcus pyogenes in the samples. The results suggest that flow cytometry may serve as a basis for an automated reliable method for the diagnosis of streptococcal infections.