Hyperspectral cytometry

vendredi 9 septembre 2011
par   G. Grégori

PRECYM et Purdue University collaborent pour développer une nouvelle génération de cytomètres capables de mieux analyser le spectre de fluorescence émis par les cellules. Très prochainement sera publié dans le journal Cytometry Part A un article présentant un dispositif qui permet de collecter 32 canaux de fluorescences :

Gregori, G., Patsekina, V., Rajwa, B., Jones, J., Ragheb, K., Holdman, C., Robinson, J.P., (2012) Hyperspectral cytometry at the single-cell level using a 32-channel photodetector. Cytometry 81A : 35-44.

Ce dispositif hyperspectral permet d’appréhender le spectre de fluorescence des particules (cellules) dans le visible, à haute vitesse. Voici le résumé de l’article :

Despite recent progress in cell-analysis technology, rapid classification of cells remains a very difficult task. Among the techniques available, flow cytometry (FCM) is considered especially powerful, because it is able to perform multiparametric analyses of single biological particles at a high flow rate–up to several thousand particles per second. Moreover, FCM is nondestructive, and flow cytometric analysis can be performed on live cells. The current limit for simultaneously detectable fluorescence signals in FCM is around 8–15 depending upon the instrument. Obtaining multiparametric measure ments is a very complex task, and the necessity for fluorescence spectral overlap compensation creates a number of additional difficulties to solve. Further, to obtain well separated single spectral bands a very complex set of optical filters is required. This study describes the key components and principles involved in building a next-generation flow cytometer based on a 32-channel PMT array detector, a phase-volume holographic grating, and a fast electronic board. The system is capable of full-spectral data collection and spectral analysis at the single-cell level.