Modifying a FC500 Flow Cytometer with Multiple Lasers, 32 fluorescence channels, and 5 angle light scattter

dimanche 25 novembre 2012
par  Aude BARANI

Gérald GREGORI in colllaboration with Paul ROBINSON and the people from the Purdue University Cytometry Labs have modified a FC500 flow cytometer (Beckman Coulter) with multiple lasers using the iFLEX-Viper from QIoptics. A 32-channel PMT was also installed in order to collect 32 channels of fluorescence., as described in the following article Grégori G. et al., DOI : 10.1002/cyto.a.21120.

In addition a multi-angle light scatter detector was mounted instead of the regular forward scatter detector. Of course, in order to collect all the parameters the electronics of the FC500 had to be supplemented with the electronics of the Gallios flow cytometer.

You are also invited to watch the video on the PUCL Web Site :
- PUCL Video-tutorial.


Modified FC500