Hyperspectral Cytometry at the Single-Cell Level Using a 32-Channel Photodetector

lundi 29 août 2011

JPEG - 19.1 ko

Zoom sur un Article :

Gregori, G., Patsekina, V., Rajwa, B., Jones, J., Ragheb, K., Holdman, C., Robinson, J.P., (2011) Hyperspectral cytometry at the single-cell level using a 32-channel photodetector. Cytometry (DOI : 10.1002/cyto.a.21120)

Despite recent progress in cell-analysis technology, rapid classification of cells remains a
very difficult task. Among the techniques available, flow cytometry (FCM) is considered
especially powerful, because it is able to perform multiparametric analyses of single bio-
logical particles at a high flow rate–up to several thousand particles per second. More-
over, FCM is nondestructive, and flow cytometric analysis can be performed on live
cells. The current limit for simultaneously detectable fluorescence signals in FCM is
around 8–15 depending upon the instrument. Obtaining multiparametric measure-
ments is a very complex task, and the necessity for fluorescence spectral overlap com-
pensation creates a number of additional difficulties to solve. Further, to obtain well-
separated single spectral bands a very complex set of optical filters is required. This
study describes the key components and principles involved in building a next-genera-
tion flow cytometer based on a 32-channel PMT array detector, a phase-volume holo-
graphic grating, and a fast electronic board. The system is capable of full-spectral data
collection and spectral analysis at the single-cell level.